Pulis' side were guilty of squandering a number of chances in a scrappy game. The Potters were not once, not twice but thrice denied by Reading's lucky yet watertight defence.
The third placed Royals eventually won the match 1-0 thanks to a first half strike from phenomenal forward Dave Kitson.
The Potters could have levelled the game in the first half when Gifton Noel-Williams' strike bounced just wide of Marcus Hahnemann's right-hand upright.
But in the second period the visitors certainly should have snatched a point.
Former Royal Carl Asaba had a stunning header athletically tipped away by Reading's American goalkeeper Hahnemann, whilst Chris Greenacre was denied a late equaliser when his goalbound shot struck City striker Ade Akinbiyi.
Pulis, who has seen his side slide down the table after an impressive start to the campaign, was somewhat down hearted after seeing his team denied at the Madejski Stadium.
"We're very disappointed not to get anything out of the game," he said, speaking after today's match.
"Both sides went for it and wanted to win and I thought we played really well. We got round the back of them and created the openings but we just lacked that cutting edge when it really mattered.
"That said, you can't legislate for the sort of fortune we had near the end when Chris Greenacre's shot is going in until it hits Ade on the line. That's just bad luck and indicative of our plight at the moment, we are just not getting those vital little breaks which can make the difference."
The Potters, who face Crewe next weekend, had been beaten 1-0 by the Royals three weeks ago in the reverse fixture at the Britannia Stadium.